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40 S6L3-33 FKKFWKWFRRF No. of experiments: 2

ID

Activity

Organism

Method
106

In vitro Preformed biofilm

S. mutans Clarke UA159 / ATCC 700610

OD600
109

In vitro Preformed biofilm

S. sanguinis NY101

OD600

41 C16-33 TRRRLFNRSFTQALGKSGGGFKKFWKWFRRF No. of experiments: 2

ID

Activity

Organism

Method
107

In vitro Preformed biofilm

S. mutans Clarke UA159 / ATCC 700610

OD600
108

In vitro Preformed biofilm

S. sanguinis NY101

OD600

42 M8-33 TFFRLFNRSGGGFKKFWKWFRRF No. of experiments: 2

ID

Activity

Organism

Method
105

In vitro Preformed biofilm

S. mutans Clarke UA159 / ATCC 700610

OD600
110

In vitro Preformed biofilm

S. sanguinis NY101

OD600

43 nisin ITSISLCTPGCKTGALMGCNMKTATCHCSIHVSK No. of experiments: 13

ID

Activity

Organism

Method
111

In vitro Adhesion (1-2h)

S. aureus ATCC 43300

Crystal violet assay
112

In vitro Preformed biofilm

S. aureus ATCC 43300

Total countable CFUs
113

In vitro Formation (3-24h)

S. aureus ATCC 43300

Crystal violet assay
246

In vitro Formation (3-24h)

S. aureus ATCC 6538

Crystal violet assay
381

In vitro Adhesion (1-2h)

S. epidermidis ATCC 35984

Safranin staining
382

In vitro Adhesion (1-2h)

S. epidermidis ATCC 35984

Safranin staining
383

In vitro Adhesion (1-2h)

S. aureus

Safranin staining
384

In vitro Adhesion (1-2h)

S. aureus

Safranin staining
385

In vitro Adhesion (1-2h)

L. monocytogenes ATCC 7644

Safranin staining
386

In vitro Adhesion (1-2h)

L. monocytogenes ATCC 7644

Safranin staining
387

In vitro Adhesion (1-2h)

L. monocytogenes ATCC 7644

Safranin staining
388

In vitro Adhesion (1-2h)

S. aureus

Safranin staining
389

In vitro Adhesion (1-2h)

S. epidermidis ATCC 35984

Safranin staining

44 indolicidin ILPWKWPWWPWRR No. of experiments: 3

ID

Activity

Organism

Method
114

In vitro Adhesion (1-2h)

S. aureus ATCC 43300

Crystal violet assay
115

In vitro Preformed biofilm

S. aureus ATCC 43300

Total countable CFUs
116

In vitro Formation (3-24h)

S. aureus ATCC 43300

Crystal violet assay

45 Cecropin A (1-7)-Melittin KWKLFKKIGAVLKVL No. of experiments: 6

ID

Activity

Organism

Method
117

In vitro Adhesion (1-2h)

S. aureus ATCC 43300

Crystal violet assay
118

In vitro Preformed biofilm

S. aureus ATCC 43300

Total countable CFUs
119

In vitro Formation (3-24h)

S. aureus ATCC 43300

Crystal violet assay
298

In vitro Preformed biofilm

P. aeruginosa

Total countable CFUs
304

In vitro Adhesion (1-2h)

P. aeruginosa

OD600
305

In vitro Formation (3-24h)

P. aeruginosa

OD600

46 HH15 KRFRIRVRVIRK No. of experiments: 1

ID

Activity

Organism

Method
120

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

47 Bac2a RLARIVVIRVAR No. of experiments: 1

ID

Activity

Organism

Method
122

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

48 1026 VQWRIRVRVIKK No. of experiments: 1

ID

Activity

Organism

Method
123

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

49 1029 KQFRIRVRV No. of experiments: 1

ID

Activity

Organism

Method
124

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

50 1036 VQFRIRVRIVIRK No. of experiments: 1

ID

Activity

Organism

Method
125

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

51 1037 KRFRIRVRV No. of experiments: 5

ID

Activity

Organism

Method
126

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay
143

In vitro Formation (3-24h)

B. cenocepacia

Crystal violet assay
144

In vitro Formation (3-24h)

L. monocytogenes

Crystal violet assay
145

In vitro Formation (3-24h)

P. aeruginosa PA14

Crystal violet assay
146

In vitro Formation (3-24h)

P. aeruginosa PAO1 / ATCC 15692

Crystal violet assay

52 HH2 VQLRIRVAVIRA No. of experiments: 1

ID

Activity

Organism

Method
127

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

53 1002 or L1002 VQRWLIVWRIRK No. of experiments: 3

ID

Activity

Organism

Method
128

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay
442

In vitro Formation (3-24h)

P. aeruginosa

Confocal microscopy-fluorescence
443

In vitro Formation (3-24h)

K. pneumoniae LM21

Confocal microscopy-fluorescence

54 1003 IVWKIKRWWVGR No. of experiments: 1

ID

Activity

Organism

Method
129

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

55 1004 RFWKVRVKYIRF No. of experiments: 1

ID

Activity

Organism

Method
130

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

56 1008 RIKWIVRFR No. of experiments: 1

ID

Activity

Organism

Method
131

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

57 HH7 VRLRIRVAVRRA No. of experiments: 1

ID

Activity

Organism

Method
132

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

58 1010 IRWRIRVWVRRI No. of experiments: 1

ID

Activity

Organism

Method
133

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

59 1011 RRWVVWRIVQRR No. of experiments: 1

ID

Activity

Organism

Method
134

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay

60 1012 or L1012 IFWRRIVIVKKF No. of experiments: 3

ID

Activity

Organism

Method
135

In vitro Formation (3-24h)

P. aeruginosa

Crystal violet assay
438

In vitro Formation (3-24h)

P. aeruginosa

Confocal microscopy-fluorescence
439

In vitro Formation (3-24h)

K. pneumoniae LM21

Confocal microscopy-fluorescence

Page 3 of 11

M. Di Luca, G. Maccari, G. Maisetta, G. Batoni BaAMPs: the database of biofilm-active antimicrobial peptides Biofouling 2015; 31(2):193-9.

Please do not hesitate to address comments or questions to: mariagrazia.diluca@nano.cnr.it or gpmaccari@gmail.com