#EXP00267

General

Accession EXP00267
Activity Formation (3-24h)
Date 2014-04-18
Administration In solution
Model in vitro
Organism Staphylococcus epidermidis ATCC 35984
Status Validated
Notes No notes

Peptide

beta6-20-G3K6

NEEGFFSARGHRPLDGGGKKKKKK

Experimental activity

Method Concentration Notes
XTT assay 100 uM No notes
Polysaccharide quantification 50 uM WGA conjugated to Alexa Fluor was used to assess the PIA content
Cell number/microscopy 50 uM Syto 9 was used to assess the amount of surface-adherent bacteria

References

Disruption of Staphylococcus epidermidis biofilm formation using a targeted cationic peptide

Christopher M. Hofmann, Kyle J. Bednar, James M. Anderson, Roger E. Marchant

2012. Journal of Biomedical Materials Research Part A 4:1061-1067